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- Title
Characterization and Antibiotic Susceptibility of E. Coli O157:h7 in Meat and Fish Sold in Major Ibadan Markets, Nigeria.
- Authors
Ayodele, Oluwafunmilayo Abosede; Deji-Agboola, Anotu Mope; Faneye, Adedayo Omotayo; Akinduti, Paul Akinniyi
- Abstract
E. coli O157:H7 is one of the major causes of foodborne illness and it is of public health importance. Thus, the aim of this study was to isolate and characterize Escherichia coli O157:H7 from meat and fish sold in Ibadan markets. A total of 400 samples comprising of raw meat (beef, goat, pig, chicken, turkey) and fish (Sardine, Titus) were purchased from major markets in different parts of Ibadan. The samples were preenriched in tryptone soya broth at 37°C for 24 hours, subcultured onto MacConkey, Blood and Sorbitol MacConkey agar plates. Thereafter, the organisms isolated were screened biochemically using Microbact GNB 12E. Antibiotic susceptibility and Double-disk synergy was performed using disc diffusion, Plate agglutination and PCR tests were used to identify E. coli O157:H7. 78 (19.5%) of the isolates obtained were identified as E. coli. The incidence of E. coli serotype O157:H7 was 6 (1.5%), 3 (50%) carries eaeA, hly, rfbE and flich7 genes, 3(50%) harboured flich7, rfbE and hly gene. The E. coli O157:H7 isolates were from beef 1 (0.8%), chevon - 1 (2.0%), pork 1 (4.0%), Sardine fish 2 (3.3%), Titus fish 1 (1.7%). 4 (66.7%) of the E. coli O157:H7 isolated were resistant to two or more antibiotics. The resistance rate was very high in Ampicillin - 66.7%, Cefuroxime - 66.7% and Gentamicin - 50.0%. The isolates were 100% sensitive to Ceftazidime and Ciprofloxacin. The isolation of E. coli O157:H7 in raw meat/fish and the existence of antimicrobial resistant isolates highlight the potential threat to public health.
- Publication
American Journal of Biomedical Sciences, 2020, Vol 12, Issue 2, p99
- ISSN
1937-9080
- Publication type
Academic Journal
- DOI
10.5099/aj200200099