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- Title
Evaluation of Flavonoid Rich Extract of Hybanthus enneaspermus in Streptozotocin-Nicotinamide Induced Diabetic Rats through Measurement of Antioxidant Enzymes (Superoxide Dismutase, Catalase, and Thiobarbituric Acid Reactive Substance), Liver Glycogen and Lipid Parameters in Liver Tissue
- Authors
Patel, DinesH K.
- Abstract
Background/Aims To search of effective compound from Hybanthus enneaspermus used in the treatment of different type of disease including diabetes by tribal people in the country. Methods The flavonoid rich fraction was isolated using ethyl acetate solvent. Different phytochemical analysis was carried out to know the chemical compositions. Male Charles Foster rats (range, 180 to 200 g) were used in this study. All the animal experiments were performed in accordance with the guidelines approved by the Ethical Committee. Effect of ethyl acetate fraction of Hybanthus enneaspermus (EHE) was evaluated at a dose of 45 and 90 mg/kg, p.o per day for 21 days in diabetic rats. Further effect of ethyl acetate fraction of EHE on blood glucose, body weight, enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and thiobarbituric acid reactive substance (TBARS) were analyzed in the supernatant of the 10% liver tissue homogenate in 0.025 M Tris-HCl buffer, pH 7.5. Lipid parameters such as total cholesterol (TC), triglycerides (TG) and high-density lipoprotein cholesterol (HDL-C) levels were determined by enzymatic methods, further low-density lipoprotein- cholesterol (LDL-C), very low-density lipoprotein-cholesterol (VLDL-C), atherogenic index (AI) and HTR ratio were also determined. Effect of EHE on glucose uptake by rat hemidiaphragm was investigated. Results The results showed that EHE contain significant amount of phenolic, tannin, flavonoid, flavonol and saponin content. All the tested lipid parameters (TC, TG, HDL-C, VLDL-C, AI, and HTR ratio) and antioxidant enzyme (SOD, CAT, and TBARS) level were significantly restored to the normal level in the EHE treated rats. Moreover, there was a significant increase in the body weight and glycogen contents in the EHE treated rats. Glucose uptake by hemidiaphragm was also enhanced in the presence of EHE. Conclusions From the obtained data it was concluded that EHE has very impressive profile as an antioxidant and lipid lowering agents.
- Publication
Gut & Liver, 2019, Vol 13, Issue 6(suppl. 1), p213
- ISSN
1976-2283
- Publication type
Academic Journal