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- Title
A Lactobacillus plantarum strain newly isolated from Chinese sauerkraut with high γ-aminobutyric acid productivity and its culture conditions optimization.
- Authors
Weiqing CHEN; Wenwen XU; Xinmo ZHENG
- Abstract
The objective of this study was to screen GABA-producing strain from traditional Chinese fermented sauerkraut and optimize culture conditions of the isolated strain for GABA production. One out of 36 strains isolated from different Chinese sauerkraut samples was obtained with high GABA production, which was identified as Lactobacillus plantarum by 16S rDNA sequence and analysis phenotypic characterization together with its morphological and physiological characterization, it was nominated as L. plantarum CWQ-7. Optimal culture conditions for growth of L. plantarum CWQ-7 and accumulation of GABA were 55g/L L-glutamate and 0.10 g/L pyridoxine hydrochloride added in modified MRS medium at initial pH 5.5 and 32°C for 66 h. Under such conditions, the GABA production reached 6. 35 g/L, with an increase of 43.6% compared to that of the original fermentation conditions. The results of the study indicated this newly isolated strain could be applied in GABA food production.
- Publication
Metallurgical & Mining Industry, 2015, Issue 9, p388
- ISSN
2076-0507
- Publication type
Academic Journal