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- Title
Cloning and sequence analysis of heat shock protein 70 gene (hsp70) from Bactrocera cucurbitae Coquillett.
- Authors
JIANG Jian-jun; WANG Feng-ying; HUANG Li-fei; CHEN Hong-song; YANG Lang; LI Lei
- Abstract
[Objective] The present experiment was conducted to clone and analyze heat shock protein 70 gene (hsp70 gene) from Bactrocera cucurbitae Coquillett to lay the foundation for further study on heat adaptability mechanism of B.cu-curbitae and making comprehensive prevention and control strategy. [Methods] Based on the amino acid sequence of insect hsp70 from NCBI, the degenerate primers were designed to amplify full-length fragment of hsp70 gene from B.cucurbitae by using RACE-PCR method. [Results] The result showed that, the obtained full-length fragment of hsp70 gene cNDA (accession number: KM112021) was 2271 bp in length, which contained a open reading frame of 1911 bp encoding 636 amino acids with three sequence signatures of hsp70 gene family and EEVD motif in C-terminal region. Therefore, the hsp70 gene of B. cucurbitae was proven to belong to cytoplasmic heat shock protein. The BLAST analysis showed that the obtained hsp70 gene had high similarity with hsp70 gene of insect from Diptera Tephritidae, which shared the highest similarity at 91%. The phylogenic tree based on the amino acid sequence showed B. cucurbitae and insects of Diptera Tephritidae could be classed into a branch, which certified that hsp70 gene was highly conserved sequence. [Conclusion] The hsp70 gene is 2271 bp in length, has characteristic of eukaryote hsp70 gene family, and shows highly conserved.
- Publication
Journal of Southern Agriculture, 2015, Vol 46, Issue 5, p800
- ISSN
2095-1191
- Publication type
Academic Journal
- DOI
10.3969/j:issn.2095-1191.2015.5.800