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- Title
YENİDEN BİRLEŞTİRİLMİŞ PROTEİNLERİN PROTEAZ KULLANILMADAN SAFLAŞTIRILMASINDA İNTEİN ARACILI AYIRMA SÜREÇLERİNİN İNCELENMESİ ÜZERİNE BİR DERLEME.
- Authors
ERMURAT, Yakup
- Abstract
In this review study, conducted researches on inteins and recombinant proteins to increase splitting activity of inteins that are utilised in recombinant protein purification processes without use of protease have been investigated. Researches on protein cleaving by use of inteins are aimed to make inteins and protein purification processes become more effective. The processes are accomplished in vivo and in vitro and intein and target protein included polyclonal Polymerase Chain Reaction (PCR) products are cloned than the expressed plasmids are fused to cloned strains. Kinetics of activity of cleaving and activity of cleaving rates were determined in different pH, temperature and filtration times. In the completed researches, protein cleaving activities of different microbial natural and engineered inteins have been investigated and among them the cleaving activity of recombinant Nostoc punctiforme (Npu) DnaE C-inteins were found to be very high. The studies are focused on the C-inteins due to their recepient abilities of the amino acid additions.
- Publication
Anadolu University of Sciences & Technology - C: Life Sciences & Biotechnology, 2017, Vol 6, Issue 2, p95
- ISSN
2146-0264
- Publication type
Academic Journal
- DOI
10.18036/aubtdc.267017