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- Title
Protective Effects of Alpha-Lipoic Acid Against Oxidative Injury in TNBS-induced Colitis.
- Authors
Şehirli, Ahmet Özer; Tatlıdede, Elif; Yüksel, Meral; Çetinel, Şule; Erzik, Can; Yeğen, Berrak; Şener, Göksel
- Abstract
Purpose: Alpha-Lipoic acid (ALA), which has been intensely investigated as a therapeutic agent for several diseases, is elucidated for its possible protective effect as a potent antioxidant on colonic inflammation. Material and Methods: Following intracolonic administration of trinitrobenzene sulphonic acid, Sprague-Dawley rats were treated orally either with saline or ALA (100 mg/kg/day), for three days. On the 4 th day, rats were decapitated and distal colon was removed for the macroscopic and microscopic damage scoring, for the measurement of malondialdehyde (MDA), glutathione (GSH) and collagen levels, myeloperoxidase (MPO) and Na + - K + -ATPase activity, luminol and lucigenin chemiluminescences (CL) and oxidant-induced DNA fragmentation. Lactate deydrogenase (LDH) activity, TNF-α, IL-1β, IL-6 and antioxidant capacity (AOC) were assayed in blood samples. Results: Colitis caused significant increases in the colonic macroscopic and microscopic damage scores, MDA, and collagen levels, MPO activity and CL values, along with a significant decrease in tissue GSH level and Na + - K + -ATP ase activity. Similarly, serum cytokines as well as LDH were elevated in the vehicle-treated colitis group as compared to control group. On the other hand, ALA treatment reversed all these biochemical indices, as well as histopathological alterations induced by TNBS. Conclusion: ALA protects the colonic tissue via its antioxidant and membrane stabilizing properties.
- Publication
Erciyes Medical Journal / Erciyes Tip Dergisi, 2009, Vol 31, Issue 1, p015
- ISSN
2149-2247
- Publication type
Academic Journal