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- Title
Detection of Chlorogenic Acid, Macranthoidin B and Dipsacoside B in FLOS LONICERAE Grown in Guizhou Province by UPLC-PDA-ELSD.
- Authors
Liucun ZHAO; Guanqing WEN; Li LI; Shuaihui ZHANG; Shiyun YE
- Abstract
[Objective] To detect chlorogenic acid, macranthoidin B and dipsaocoside B in FLOS LONICERAE grown in Guizhou Province by UPLC-PDA-ELSD, and to investigate the quality of FLOS LONICERAE grown in Guizhou Province. [Methods] ACQUTTYUPLC BEH C18 chromatographic column (2.1 mm × 100 mm, 1.7 μm) was adopted; mobile phase was acetonitrile (A) - 0.4% acetic acid water (B); gradient elution was as follows: 0-2 min, 7% A; 2-2.3 min. 7%-28% A; 2.3-10 min, 28% A; 10-10.5 min, 28%-7% A; gradient flow rate was as follows; 0-2 min, 0.35 mL/min; 2-2.3 min, 0.35-0.25 mL/min; 2.3-2.6 min, 0.25-0.15 mL/min; 2.6-2.8 min, 0.15-0.1 mL/min; 2.8-4 min, 0.1-0.2 mL/min; 4-4.3 min, 0.2-0.3 mL/min; 4.3-4.5 min, 0.3-0.35 mL/min; 4.5-10.5 min, 0. 35 mL/min; PDA (ultraviolet detector) was used to detect the ehlorogenie acid (330 nm wavelength); ELSD (evaporative light-scattering detector) was used to detected the macranthoidin B and dipsacoside B; temperature of the drift tube was 60 °C ; sprayer was 48 °C; gas pressure was 137.9 kPa; and column temperature was 50 °C. [Results] Chlorogenie acid, macranthoidin B and dipsaooside B showed good linear relationship within the ranges of 0.3045-1.5225 μg/mL (R2 = 0.9994), 0.449-1.4368 μg/mL (R2 = 0.9993) and 0.0446-0.223 μg/mL (R2= 0.9994), respectively. [Conclusions] This method could accurately detect the contents of chlorogenie acid, macranthoidin B and dipsaeoside B in FLOS LONIGERAE grown in Guizhou Province, effectively shorten the measurement time and save reagents, so that it was suitable to detect the quality of FLOS LONICERAE grown in Guizhou Province.
- Publication
Medicinal Plant, 2015, Vol 6, Issue 7/8, p17
- ISSN
2152-3924
- Publication type
Academic Journal