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- Title
Analysis of Amino Acids in Houttuynia Cordata Thunb. by High Performance Liquid Chromatography-Diode Array Detector ( HPLCDAD).
- Authors
Xuan FENG; Zhannan YANG; Zhu ZHENG; Juan HU; Xiaofang WEI; Honghao HUO; Cheng ZHAO; QuJing ZHANG
- Abstract
[Objectives] The biologically active compounds of Houttuynia cordata Thunb. as a medicinal and edible plant are well documented. However, amino acid as an important indicator of the value of H. cordata is little reported. [Methods] Amino acids of the H. cordata were analysed by pre-column derivatization with 2,4-dinitrochlorobenzne(CDNB) coupled to HPLC/DAD. The optimized conditions of the amino acids derivatized by CDNB were followed: 110 ℃ of hydrolysis temperature, 1∶12 of the equivalent proportion between amino acid and CDNB, 80 min of derivatization reaction time and 80 ℃ of derivatization reaction temperature. The method was used to determine the amino acids of H. cordata samples from 11 different geographical sites in Guizhou. [Results] The results suggested that the contents of 17 amino acids were quite variable at different H. cordata samples. [Conclusions] This study will provide a basis for the quality evaluation of H. cordata nutrition.
- Publication
Medicinal Plant, 2019, Vol 10, Issue 2, p28
- ISSN
2152-3924
- Publication type
Academic Journal
- DOI
10.19600/j.cnki.issn2152-3924.2019.02.009