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- Title
Combined Therapy of Contused Rat Spinal Cord with Glial Cell Line-Derived Neurotrophic Factor (GDNF) and Motoneurons Transdifferentiated from Adipose-Derived Stem Cells.
- Authors
Abdanipour, A.
- Abstract
Objective: Cell transplantation is a potential treatment strategy for animal models of spinal cord injury (SCI). The purpose of this study was to evaluate combination therapy with glial cell line-derived neurotrophic factor (GDNF) and transplantation of transdifferentiated motoneuron- like cells (MNLCs). Materials and Methods: Adipose-derived stem cells (ADSCs) were preinduced with selegiline and then induced with Shh and all-trans retinoic acid (RA). The treated animals were evaluated using the Basso, Beattie, and Bresnahan (BBB) test, relative difference coefficients (RDC), immunohistochemical staining, and morphometry (for 12 weeks after injury). Results: The induced MNLCs were immunoreactive to Oligo2 and choline acetyltransferase. The results of the BBB test showed a significant improvement in the group that received combination treatment with MNLCs and GDNF, as compared with the untreated group. Histological assessments showed that combination therapy reduced cavity formation and increased cell density at the lesion epicenter, and regions rostral and caudal to the lesion. Conclusion: The conclusion of our study is that combination treatment with MNLC transplantation and GDNF ameliorates locomotor dysfunction in rats with SCI
- Publication
Cell Journal (Yakhteh), 2013, Vol 15, Issue Sup 1, p20
- ISSN
2228-5806
- Publication type
Academic Journal