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- Title
Scrophularia striata with antiinflammatory and neuroprotective properties.
- Authors
Azadmehr, Abbas; Hajiaghaee, Reza; Amirghofran, Zahra
- Abstract
Objectives: In this study, the neuroprotective effects of extracts from Scrophularia striata, a medicinal plant growing in Northeastern Iran, against neurocytotoxicity induced by oxidative stress were evaluated in PC-12 cell line. Materials and Methods: Different concentrations (10, 50, 100 and 200 µg/ml) of the extracts were injected in the PC-12 cell line and then the cells were treated with H2O2 to induce oxidative stress and neurotoxicity. Survival of the cells, reactive oxygen species (ROS) production and apoptosis were measured, using MTT assay, fluorescent probe, 2', 7'-dichlorofluorescein diacetate (DCFH-DA) and annexin V/propidium iodide, respectively. Moreover, 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to evaluate the antioxidant capacity of plant extracts. Results: Phytochemical assay by thin-layer chromatography showed that components such as phenolic compounds, phenylpropanoids and flavonoids can be found in S. striata extracts. The extracts at concentrations of 50-200 µg/ml protected PC-12 cells from H2O2-induced toxicity. The survival of the cells at an extract concentration of 200 µg/ml was 64%, compared to 48% survival in cells treated by only H2O2 (p<0.001). Moreover, the extracts showed antioxidative properties and decreased the number of apoptotic cells. Conclusion: Overall, these finding indicated the ability of S. striata in reducing ROS production and cell apoptosis. Additionally, the presence of neuroprotective agents in this plant was suggested.
- Publication
Avicenna Journal of Phytomedicine, 2015, Vol 5, p15
- ISSN
2228-7930
- Publication type
Academic Journal