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- Title
Neuroprotective effect of silymarin on acrylamide-induced neurotoxicity in both in-vitro and in-vivo assessments.
- Authors
Mehri, Soghra; Dadesh, Qutaiba; Hosseinzadeh, Hossein
- Abstract
Objectives: Acrylamide is a well-known toxic chemical, which has broad applications in several industries. Different mechanisms including induction of oxidative stress and apoptosis are involved in acrylamide(ACR)-induced toxicity. Regarding the potent antioxidant properties of silymarin, the important constituent of Silybum marianum, the possible protective effect of silymarin against ACR-induced toxicity on Wistar rats and PC12 cells was evaluated. Materials and Methods: Animals were treated with ACR (50 mg/kg, ip) for 11 days to induce toxicity. Silymarin (40, 80 and 160 mg/kg, ip) was used during the treatment with ACR. At the end of treatment, the behavioral index was evaluated. For the in-vitro assay, PC12 cells were used as a suitable in-vitro model. Cells were exposed to different concentrations of silymarin for 24 hours, then ACR with IC50 concentration (5 mm) was added to the cells. Finally, cell viability was determined using MTT assay. Results: In Wistar rats, exposure to ACR significantly induced severe gait abnormalities, but treatment with silymarin (160 mg/kg) reduced ACR- induced neurotoxicity in animals. ACR decreased cell viability, and pre-treatment with silymarin significantly decreased ACR- induced cytotoxicity in a dose-dependent manner. Conclusion: The neuroprotective effect of silymarin in this study can be due to inhibition of oxidative stress, since silymarin has shown antioxidant effects invarious studies.
- Publication
Avicenna Journal of Phytomedicine, 2015, Vol 5, p86
- ISSN
2228-7930
- Publication type
Academic Journal