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- Title
Evaluating concentrated smear technique and manual MGIT system for detection of AFB in smear-negative presumptive cases of pulmonary tuberculosis for use in resource-limited settings.
- Authors
Jamali, Sana; Richa; Srivastava, Vinod K.
- Abstract
Background: Tuberculosis is a major public health problem in India. According to RNTCP guidelines, all efforts are to be made to diagnose tuberculosis on the basis of detection of Acid-Fast Bacilli (AFB)/ Macrobacterium Tubercle Bacilli (MTB) in the clinical specimen; hence all those cases where AFB/ MTB have not been detected are presumptive cases of tuberculosis. Aim and Objectives: To detect AFB/ MTB in the sputum of clinico-radiologically presumptive cases of pulmonary tuberculosis using direct ZN microscopy, Modified Petroff's method followed by ZN microscopy & MGIT liquid culture method. Materials and Methods: A total of 94 sputum samples which were negative for AFB by direct ZN microscopy at the Designated Microscopy Centers (DMC) of the institute, were subjected to Modified Petroff's method followed by microscopy & MGIT liquid culture test to find out any additional yield of bacteriologically confirmed disease. Result: Among all the specimens, Modified Petroff's method added 10 AFB positive & MGIT method could add 30 cases (including those found positive by Modified Petroff's method). Conclusion: Modified Petroff's method followed by ZN microscopy & MGIT test for mycobacterial culture can improve case detection.
- Publication
Indian Journal of Community Health, 2019, Vol 31, Issue 2, p231
- ISSN
0971-7587
- Publication type
Academic Journal