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- Title
PROTECTIVE EFFECT OF QUERCETIN AND LUTEOLIN ON LPS-INDUCED OXIDATIVE STRESS IN RAW 264.7 CELLS.
- Authors
KURPIOS-PIEC, DAGMARA; CZECZOT, HANNA; PODSIAD, MAŁGORZATA
- Abstract
Numerous studies suggest that flavonoid dietary intake may be beneficial for the prevention and treatment of several chronic diseases associated with inflammation. These natural compounds can be useful as adjuvant therapy for their potential anti-inflammatory effect, associated with antioxidant activity. Thus, the aim of this study was to investigate the antioxidant potential of two flavonoids (quercetin and luteolin) in LPS-induced inflammation. We investigated the influence of flavonoids (quercetin and luteolin) on total glutathione (GSHt) and oxidized glutathione (GSSG) level, the ratio of reduced to oxidized glutathione (GSH/GSSG) as well as on the activity of glutathione-related enzymes: seleniumdependent glutathione peroxidase (Se-GPx), non-selenium-dependent glutathione peroxidase (non-Se- GPx) and glutathione reductase (GR) in LPS-stimulated RAW 264.7 cells. Our study showed that in the LPS stimulated macrophages both flavonoids at lower concentrations (2.5 and 5 µg/mL) demonstrated antioxidant properties, observed as increased GSHt level, GSH/GSSG ratio, as well as increased GR activity. Quercetin demonstrated higher antioxidant potential than luteolin, which can be explained by its different structure and/or bioavailability. On the contrary, both quercetin (10 µg/mL) and luteolin (5 and 7.5 µg/mL) revealed also slight prooxidative properties observed as elevated GSSG levels in LPS stimulated macrophages. These effects may be associated with increased ROS production in RAW 264.7 cells. The antioxidant activity of flavonoids (quercetin, luteolin) indicates that as dietary supplements or pharmaceuticals (pharmaceutical preparations) they can be used as potential agents in the prevention and / or treatment of inflammatory diseases.
- Publication
Acta Poloniae Pharmaceutica, 2021, Vol 78, Issue 4, p527
- ISSN
0001-6837
- Publication type
Academic Journal
- DOI
10.32383/appdr/140329