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- Title
Sulglycotide Effect on the Proteolytic and Lipolytic Activities of Helicobacter pylori toward Gastric Mucus.
- Authors
Piotrowski, J.; Czajkowski, A.; Yotsumoto, F.; Slomiany, A.; Slomiany, B. L.
- Abstract
Objectives: Infection with Helicobacter pylori is now recognized as a major factor in the etiology of gastric disease, and among the detrimental effects this bacterium exerts on the mucosal integrity is the elaboration of extracellular protease and lipase enzymes capable of mucus protein and lipids degradation. We present here evidence that the activities of these enzymes are inhibited by an gastroprotective agent, sulglycotide. Methods: The grown colonies of bacterium were washed with saline, filtered through sterilization filter, and the filtrate used as the enzyme source. Results: In the absence of sulglycotide, the H. pylori protease caused extensive degradation of human gastric mucus, while free fatty acids, glycerol monooleate and lysopbosphatidylcholine were produced by the action of H. pylori lipase and phospholipase A enzymes. Introduction of sulglycotide to the incubation systems led to the reduction in the rate of mucus protein and lipid degradation. The rate of proteolysis inhibition was proportional to sulglycotide concentration up to 45 μ/ml, at which point a 43% reduction in mucus degradation was attained, whereas the maximum inhibition of lipase (39%) and phospho-Upase A (98%) activities occurred at a sulglycotide concentration of 1OO μ/ml. Conclusions: This study indicates that sulglycotide is capable of counteracting the mucolytic activity of H. pylori and thus may be of value in tbe therapy of H. pylori-associated gastric diseases.
- Publication
American Journal of Gastroenterology (Springer Nature), 1994, Vol 89, Issue 2, p232
- ISSN
0002-9270
- Publication type
Academic Journal