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- Title
Lipolytic Activity of Campylobacter pylori: Effect of Colloidal Bismuth Subcitrate (De-Nol).
- Authors
Slomiany, B. L.; Kasinathan, C.; Slomiany, A.
- Abstract
Infection by <em>Campylobacter pylori</em> appears to play a major role in the etiology of gastric disease, but the nature of impairment evoked by this pathogen in gastric mucosal defense is not well understood. We present here evidence that the extracellular material elaborated by this microorganism exhibits lipolytic activity capable of gastric mucosal lipid degradation. The colonies of bacteria, cultured from antral mucosal biopsy specimens of patients undergoing endoscopy, were washed with saline, passed through a sterilization filter, and the filtrate was examined for lipase and phospholipase activities. By following the degradation of glycerol trioleate and dipalmitoyl phosphatidylcholine, we established the presence of lipase and phospholipase A enzymes. The major product of the triglyceride degradation was glycerol monooleate, while lysophosphatidylcholine resulted from the degradation of phosphatidylcholine. The lipolytic activity of <em>C. pylori</em> filtrate was inhibited by an antiulcer drug, De-Nol, which at 150 µg/ml caused a 21% reduction in lipase activity and a 60% reduction in the activity of phospholipase A. The results suggest that De-Nol is capable of preventing degradation of mucosal lipids by <em>C. pylori</em> lipases and, hence, helps to preserve the mucosal integrity.
- Publication
American Journal of Gastroenterology (Springer Nature), 1989, Vol 84, Issue 10, p1273
- ISSN
0002-9270
- Publication type
Academic Journal