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- Title
Depot-Specific Gene Expression of Lipolytic Enzymes in Human Adipose Tissues: Association with Adiposity and Plasma Lipids.
- Authors
Nagashima, Shuichi; Yagyu, Hiroaki; Takahashi, Nirei; Takahashi, Manabu; Tsuchida, Takeshi; Kurashina, Tomoyuki; Tanaka, Yasumichi; Tazoe, Fumiko; Monden, Junko; Inaba, Toshihiro; Nagasaka, Shoichiro; Okada, Koji; Ishibashi, Shun
- Abstract
Adipocyte lipolysis is mediated by multiple lipolytic enzymes that include hormone-sensitive lipase (HSL), adipose triacylglycerol lipase (ATGL), and TG hydrolase (TGH) family. The objective of the study was to investigate expression levels of these genes in various depots of human adipose tissue and their relationship to obesity/insulin resistance. We collected subcutaneous, mesenteric and omental adipose tissues from 28 subjects who underwent abdominal surgery and measured mRNA levels of the above genes. Depot-specific difference was observed only in ATGL, which was expressed 1.5-fold higher in omentum than subcutaneous adipose tissue. Subcutaneous and omental TGH mRNA levels were positively correlated with abdominal fat area (R = 0.49,p<0.05 and R= 0.46;p= 0.053 respectively) as well as with serum LDL cholesterol levels (R = 0.42,p < 0.05 and R= 0.71, p < 0.05 respectively). Mesenteric TGH mRNA levels were positively correlated with serum TG levels (R= 0.89; p < 0.01). Subcutaneous HSL mRNA levels were negatively correlated with adiposity and insulin resistance (R= -0.65, p < 0.05 and R= -0.49,p < 0.05 respectively). Mesenteric ATGL mRNA levels were positively correlated with serum TG levels CR= 0.69; p < 0.05). Our conculusion based upon the results is that. ATGL may mediate the high lipolytic activities in visceral adipose tissue and adiposity may facilitate lipolysis primarily by up-regulating TGH gene expression in human adipose tissue.
- Publication
Diabetes, 2007, Vol 56, pA467
- ISSN
0012-1797
- Publication type
Academic Journal