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- Title
VALIDATION OF NEW LIQUID CHROMATOGRAPHIC METHOD FOR NATURALLY ISOLATED QUERCETIN AND ITS COMMERCIAL APPLICATION TO AYURVEDIC FORMULATION.
- Authors
Dinnimath, B. M.; Jalalpure, S. S.
- Abstract
Quercetin was isolated from Aerva lanata (L) and investigated for method development. A simple, accurate, reproducible method was developed using RP-HPLC. Commercial formulation of Quercetin 250mg capsules (Quercetin Complex-Solgar, USA) was used followed by an Ayurvedic proprietary medicine for the study. The chromatographic separation was performed on Shiseido Capcell Pak C18 column (250 X 4.6 mm, 5 µm), mobile phase developed was 0.3% formic acid:acetonitrile:methanol (40:20:40) at the flow rate of 10µL/min at 220 C. The detector used was PDA with the detection wavelength of 370 nm. RT was 4.9mn with LOD-0.816 µg/ml and LOQ-2.473 µg/mL. The correlation coefficient was 0.992%. The linearity range was observed in the concentration range of 2-20 µg/mL and the recovery was found to be 99.91 to 102.65 %. Finally, the method applied to KACHCHNAR Ayurvedic formulation justified with characteristic peak for identifying quercetin in it.
- Publication
Indian Drugs, 2019, Vol 56, Issue 6, p46
- ISSN
0019-462X
- Publication type
Academic Journal
- DOI
10.53879/id.56.06.11496