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- Title
Mechanism of Lycium barbarum polysaccharides on primary cultured rat hippocampal neurons.
- Authors
Zhao, Peng; Ma, Ning-Tian; Chang, Ren-Yuan; Li, Yu-Xiang; Hao, Yin-Ju; Yang, Wen-Li; Zheng, Jie; Niu, Yang; Sun, Tao; Yu, Jian-Qiang
- Abstract
Lycium barbarum polysaccharides (LBP) have been reported to have a wide range of beneficial effects including neuroprotection, anti -aging and anticancer. However, the anti -inflammation mechanism of LBP on primary cultured rat hippocampal neurons injured by oxygen-glucose deprivation/reperfusion (OGD/RP) is incompletely understood. We investigate the neuroprotective effects of LBP on neonatal rat primary cultured hippocampal neurons injured by OGD/RP with different approaches: MTT assay was used to detect cell viability, lactate dehydrogenase leakage was used to detect neuronal damage, formation of reactive oxygen species was determined by using fluorescent probe DCFH-DA. Hoechst 33,342 staining and TUNEL staining were used to determine the cell apoptosis. JC-1 was used to evaluate loss of mitochondrial membrane potential (MMP). The fluorescence intensity of [Ca] in hippocampal neurons was determined by laser scanning confocal microscopy. The expression of various apoptotic markers such as TLR4, IκB, IL-6 and NF-κB were investigated by RT-PCR and western blot analysis. Results from each approach demonstrated that LBP increased the cell abilities and decreased the cell morphologic impairment. Furthermore, LBP increased MMP but inhibited [Ca] elevation and significantly suppressed overexpression of NF-κB, IL-6 TLR4 and increased IκB expression.
- Publication
Cell & Tissue Research, 2017, Vol 369, Issue 3, p455
- ISSN
0302-766X
- Publication type
Academic Journal
- DOI
10.1007/s00441-017-2648-2