We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DNA barcoding of Epimedium elatum (Berberidaceae), a rare endemic plant from the Western Himalayan region of Pakistan.
- Authors
Umer, Shakila; Muhammad, Khushi; Majid, Abdul; Ur Rehman, Shafee; Azeem, Ayesha; Ahmad, Israr; Ud-Din, Aziz
- Abstract
Epimedium elatum (Morren & Decne) belonging to the family Berberidaceae is a rare medicinal plant endemic to the Western Himalayas and is being rare species, it is an ideal case for DNA barcoding. The present study was aimed at molecular identification, authentication, and development of reference DNA barcodes for Epimedium elatum collected from the Western Himalayas of Pakistan. We investigated four reference DNA barcode markers namely, rbcL, matK, trnH-psbA, and ITS for molecular analysis of Epimedium elatum. Three out of the four DNA barcode markers showed 100% amplification and sequencing success rate; matK was the only exception. The nucleotide sequence data were analyzed using Sequencher, BLAST, Muscle alignment, BioEdit, and MEGA6 software. The ITS and TrnH-psbA sequences showed significant similarity to a related species of the genus Epimedium, while rbcL showed low discriminatory power. Further analysis revealed that all barcodes could clearly distinguish Epimedium elatum from its closely related species. We concluded that all three barcodes are powerful tools for the identification of Epimedium elatum.
- Publication
Pakistan Journal of Agricultural Sciences, 2022, Vol 59, Issue 5, p817
- ISSN
0552-9034
- Publication type
Academic Journal
- DOI
10.21162/PAKJAS/22.320