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- Title
RAPD-PCR IS A GOOD DNA FINGERPRINTING TECHNIQUE TO DETECT PHYLOGENETIC RELATIONSHIPS AMONG STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT SOURCES IN HILLA CITY, IRAQ.
- Authors
Amalraqibshamran; Shaker, Zinahhadi; Al-Awsi, Ghaidaa Raheem Lateef; Khamis, Ammar S.; Tolaifeh, Zainab A.; Jameel, Zahraa Isam
- Abstract
Staphylococcus aureus (sometimes referred to as "golden staph") is round-shaped, gram-positive bacteria, these bacteria belongs to Firmicutes, also, it can be considered as a member of normal flora of the body, it can be frequently found within skin, respiratory tract, and the nose. Usually, it is positive for nitrate and catalase reduction. To characterizes S. aureus strains that are widespread in patients in Hilla town, 60 clinical and environment samples were collected from wounds, skin, nails and urinary tract infection taken from general hospitals of Hilla town. Ways for isolation and characteristic S. aureus based mostly upon culture ways as well as biochemical tests, a complete of 17 amplified deoxyribonucleic acid fragments from 250 to 1K bp) were discovered using the two random primers and every of primer that productive giving amplification bands disclosed completely different genetic patterns. RAPD-PCR analysis verified to be of excellent value in coming up with a range of molecular deoxyribonucleic acid fingerprintg based mostly on medical specialty studies that focuses on the identification and characterization of S.aureus.
- Publication
Biochemical & Cellular Archives, 2018, Vol 18, p1157
- ISSN
0972-5075
- Publication type
Academic Journal