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- Title
EFFECT OF AQUEOUS FRUIT EXTRACT OF MIMUSOPS ELENGI ON SPERM MORPHOLOGY, ANTIOXIDANT VALUES AND APOPTOTIC GERM CELLS IN MALE MICE .
- Authors
Singh, Nidhi; Singh, Shio K.
- Abstract
Mimusops elengi have multifactorial properties such as antibacterial, antifungal, gastroprotective, cytotoxic and antifertility effect. The aim of the present study was to investigate the possible mechanisms of antifertility action of Mimusops elengi in male laboratory mouse. Mice of the Parkes (P) strain were orally administered aqueous fruit extract of Mimusops elengi in doses of 200, 400 and 600 mg/kg body weight/day, for 35 days. To find the effect of treatment on Sperm morphology, lipid peroxidation (LPO), antioxidants (SOD and catalase) and apoptosis via TUNEL and Western blot of caspase-3 were evaluated. Results showed a significant increase in frequency of morphologically abnormal spermatozoa, in LPO level and marked decrease in SOD and catalase enzymes activities were noted in the testis of mice treated with 600 mg dose of the extract. Treatment also had adverse effect on expressions of TUNEL and Western blot analysis of caspase 3 also showed that mimusops treatment induced apoptosis in the testis. By 56 days of treatment withdrawal, however, the above parameters recovered to control levels. Our results in P mice thus suggest that Mimusops treatment causes reversible suppression of spermatogenesis by inducing apoptosis in testicular germ cells, resulting from adverse effects of treatment on oxidative status in the testis as one of the mechanism by which the extract produced antifertility efficacy.
- Publication
Biochemical & Cellular Archives, 2021, Vol 21, Issue 1, p865
- ISSN
0972-5075
- Publication type
Academic Journal