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- Title
Screening of Cytotoxic Activity of Hematite (a-Fe<sub>2</sub>O<sub>3</sub>) Nanoparticles from Butea monosperma on MCF-7 Cells.
- Authors
Pradhan, Debasish; Pradhan, Shaktiprasad; Behera, Bandana; Samantaray, Adyasa
- Abstract
Background: Hematite (a-Fe2O3) has been used as an antimicrobial and disinfectant agent. Nevertheless, there are limited data about antitumor potential. This study was focused on investigating cytotoxic effects of Hematite (a-Fe2O3) from Butea monosperma flower extract on MCF -7 breast cancer cells and its mechanism of action. Materials and Methods: Thus, a green method was created for the synthesis of Hematite (a-Fe2O3) using an aqueous extract of B. monosperma flower. Synthesis of Hematite (a-Fe2O3) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2',7'-dichlorofluorescein diacetate and intracellular calcium (Cai2+) was evaluated with probe flu3-AM. Cells were treated with different concentrations of Hematite (a-Fe2O3) (1, 3, 6, 10, 15, 25, 50, and 100 µg/mL). Results: The results showed that Hematite (a-Fe2O3) hindered cell growth in a dose-dependent manner. Hematite (a-Fe2O3) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai2+ (half-maximal inhibitory concentration 52 ± 3.14). Conclusion: In conclusion, the results of this preliminary study demonstrated that Hematite (a-Fe2O3) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.
- Publication
Pharmacognosy Research, 2021, Vol 13, Issue 3, p260
- ISSN
0976-4836
- Publication type
Academic Journal
- DOI
10.4103/pr.pr_115_19