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- Title
ID 82. In vitro Adipogenic Potential and Glucose Uptake Stimulatory Effect of the Terpenoids Isolated from Tetracera indica Merr.
- Authors
Ahmed, Qamar Uddin; Hasan, Md. Mahmudul; Siddiqui, Mohammad Jamshed
- Abstract
Introduction: Mempelas paya (Tetracera indica Merr.) is used in folk medicines for the treatment of different diseases including diabetes and its related infirmities in Malaysia. This plant has been reported to contain terpenoids and flavonoids as major bioactive constituents. Flavonoids from this plant have earlier been reported for their antidiabetic potential. Objectives: The aim of this study was to evaluate the in vitro antidiabetic potential of non-polar compounds to ascertain whether they also contribute antidiabetic effect to mempelas paya. Materials and Methods: As non-polar compounds, two terpenoids viz. betulinic acid and stigmasterol were isolated from stems ethanol extract through repeated silica gel column chromatography and their structures were characterised NMR spectroscopy. In vitro antidiabetic effect was investigated through 3T3-L1 adipocytes and fluorescence glucose (2-NBDG) uptake assays. MTT viability assay was performed on 3T3-L1 pre-adipocytes to confirm the safe concentrations of both compounds. Finally, both terpenoids were subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. The cells were treated in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Rosiglitazone was used as standard. Results: Betulinic acid and stigmasterol induced significant (P<0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Furthermore, both terpenoids also exhibited significant (P<0.05) glucose uptake activity. Conclusion: This study is reporting the in vitro 2-NBDG uptake evaluation of stigmasterol and betulinic acid for the first time. The present study demonstrated that the non-polar compounds isolated from the mempelas paya do possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds.
- Publication
Journal of Pharmacy & Bioallied Sciences, 2020, Vol 12, p879
- ISSN
0976-4879
- Publication type
Academic Journal