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- Title
ANALYTICAL METHOD DEVELOPMENT OF NUTRACEUTICAL: UMBELLIFERONE.
- Authors
Malik, Anuj; Kushnoor, Ashok; Saini, Vipin; Singhal, Sarita; Kumar, Sharad; Chand Yadav, Yogesh
- Abstract
Coumarins, an old class of compounds, are naturally occurring benzopyrene derivatives. A lot of coumarins have been identified from natural sources, especially green plants. Coumarins have attracted intense interest in recent years because of their diverse pharmacological properties. Phytochemical investigation on the fruit of Aegle marmelos (Rutaceae) has resulted in the development of analytical method for Umbelliferone. The present study was undertaken to develop a validated, rapid, simple and low-cost ultraviolet (UV) spectrophotometric method for estimating Umbelliferone in dosage preparations. UV spectrophotometric analysis was performed spectrophotometrically at a pre-determined λ_max of 324 nm with distilled water (using ethanol as co-solvent) as diluent/blank. The method was validated for linearity, accuracy, precision, reproducibility, and specificity as per International Conference on Harmonization (ICH) guidelines. The method was also used in the determination of the content of umbelliferone in formulations of umbelliferone. The regression data for the calibration plots exhibited good linear relationship (R² = 0.9991) over a concentration range of 0.5-6.5 μg/ml and the linear Regression equation was y=0.169 x + 0.023. Mean recovery accuracy was 96.9 %, which was not significantly different from the expected value (p = 0.05), while coefficient of variation (CV) for both intra-day and inter-day was < 7 %. The method was specific for umbelliferone in the presence of common excipients, and when it was applied to formulations, umbelliferone content was 97.89 ± 3.08 and 99.05 ± 1.99 %, respectively, of calculated claim. The proposed method gave good validation results and the statistical analysis performed proved that the method is precise, accurate and reproducible, and hence can be employed for routine analysis of umbelliferone in bulk and commercial formulations.
- Publication
Pharma Science Monitor, 2012, Vol 3, Issue 1, p67
- ISSN
0976-9242
- Publication type
Academic Journal