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- Title
Protective effect of Ganoderma leucocontextum polysaccharide on exercise-induced fatigue in mice based on Keap1/Nrf2/HO-1 signal pathway and oxidative stress effect.
- Authors
TIAN Xing; LU Congcong
- Abstract
Objective : To study the anti-fatigue mechanism of white meat Ganoderma leucocontextum polysaccharide on high-intensity training mice. Method: Mice were randomly divided into blank control group, model control group and white meat Ganoderma leucocontextum polysaccharide low, medium and high treatment groups. The exercise- induced fatigue model of mice was established by treadmill training. 10, 50 and 100 mg/mL polysaccharide were continuously given by intragastric administration, and the other groups were intragastrically administered the same dose of sterile saline solution. After 4 weeks of continuous training, climbing rod experiments were performed, and rod climbing time of mice was recorded. Testosterone (TTS), corticosterone (CTC), blood urea nitrogen (BUN), blood lactate (BLA), liver glycogen (HG), muscle glycogen (MG), malondialdehyde (MDA) content and peroxidation in mice Hydrogenase (CAT), superoxide dismutase (SOD), glutathione superoxide dismutase (GSH-Px) and other activities were measured. Western blot was used to detect of Keap-1, Nrf2, HO-1 in mouse skeletal muscle protein expression. Result : The rod climbing time of mice in the low, medium and high polysaccharide treatment groups gradually increased significantly (P < 0.05). At the same time, the content of TTS in the serum of mice gradually increased, the content of CTC decreased gradually, and the reserves of HG and MG gradually increased. In addition, content of MDA in serum and liver tissue of mice in low, medium and high dose polysaccharide groups were decreased significantly (P < 0.05), and the enzyme activities of CAT, SOD and GSH-Px were increased significantly (P < 0.05). Compared with the blank control group, the protein expression level of Keapl in the model group was significantly high (P < 0.05), and the protein expressions of Nrf2 and HO-1 were down-regulated. The protein expression levels of Keap1 in the group decreased in turn, while the protein expression levels of Nrf2 and HO-1 increased. Conclusions : The protective effect of Ganoderma leucocontextum polysaccharides on exercise-induced fatigue in mice may be related to the enhancement of antioxidant effect of mouse skeletal muscle cells and the influence of Keap1/Nrf2/HO-1 signaling pathway.
- Publication
China Food Additives, 2023, Vol 34, Issue 1, p127
- ISSN
1006-2513
- Publication type
Academic Journal
- DOI
10.19804/j.issn1006-2513.2023.01.015