We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The best concentration and the best time of lactoferrin for the stimulation of osteoblast proliferation and differentiation.
- Authors
LIN Ying; CHEN Xiaxia; HOU Jianming; YAO Qionghua
- Abstract
Objective To explore the optimal concentration of the lactoferrin (LF) for the stimulation of rat osteoblast proliferation and the best time to promote osteoblast differentiation using 1000µg/ml lactoferrin. Methods Primary rat osteoblasts were harvested with mixed enzyme from neonatal calvaria of Sprague Dawley rats and then cultured. After the treatment with different concentrations of LF (0, 0.1, 1, 10, 100, 200, 400, 600, 800, and 1000 µg/mL) for 1, 3, 5, and 7 days, the cell proliferation was analyzed using CCK-8 methods. After the treatment with LF of 1000µg/mL for 7, 14, and 21days, alkaline phosphatase staining method was used to evaluate the ALP activity of the osteoblast. Results 1) The results of cell proliferation with CCK-8 method showed that below 100 µg/mL concentration of LF, OD value increased as the concentration increased. However, OD value decreased with the concentration of LF increased using over 100 µg/mL concentration of LF. 2) The results of ALP staining showed that the highest ALP activity was achieved on the 21st day using 1000 µg/mL concentration of LF. Conclusion The best concentration of LF for rat osteoblast proliferation is 100 µg/mL. LF of 1000 µg/mL promotes osteoblast differentiation in a time dependent manner.
- Publication
Chinese Journal of Osteoporosis, 2015, Vol 21, Issue 2, p142
- ISSN
1006-7108
- Publication type
Academic Journal
- DOI
10.3969/j.issn.1006-7108.2015.02.004