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- Title
Determination of Chlorogenic Acid, Macranthoidin B and Dipsacoside B in Flos Lonicera by HPLC-ELSD.
- Authors
Fenglai LU; Haiying JIANG; Yueyuan CHEN; Jianzhang WU; Jinlei LIU; Dianpeng Ll
- Abstract
[Objective] A method for the determination of chlorogenic acid, macranthoidin B and dipsacoside B in Flos Lonicera by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. [Method] The separation was carried out using a ZORBAX SB-C18 chromatographic column (4.6 mmx250 mm, 5 "m), with a simple mobile phase of acetonitrile and 0.1% formic acid. Evaporative light scattering detector was employed. [Result] The result showed that chlorogenic aicd, macranthoidin B and dipsacoside B showed good linearities in the ranges of 3.00-6.40, 4.24-9.33 and 0.40-2.00 μg with correlation coefficient of 0.999, 0.993 and 0.999, respectively, [inclusion] The method has the advantages of simple operation, good accuracy, repeatability and separation effect and high specificity and is suitable for overall quality control of Flos Lonicera. Key words Flos Lonicera; Chlorogenic acid; Macranthoidin B; Dipsacoside B; HPLC-ELSD.
- Publication
Agricultural Science & Technology, 2016, Vol 17, Issue 1, p26
- ISSN
1009-4229
- Publication type
Academic Journal