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- Title
Separation and Purification of Macranthoidin B and Dipsacoside B from Flos Lonicerae by HP-20 and HP-SS Macroporous Resin.
- Authors
Fenglai LU; Huan WEI; Lei WANG; Xiaojie YAN; Yueyuan CHEN; Dianpeng LI
- Abstract
[Objective] This study was conducted to develop a method for rapidly separating macranthoidin B and dipsacoside B from Flos Lonicerae. [Method] HP-20 and HP-SS macroporous resin were applied to separate and purify macranthoidin B and dipsacoside B from Flos Lonicerae. The extract of Flos Lonicerae was first loaded onto an HP-20 column to enrich saponins, which were then separated by an HP-SS macroporous resin column to get pure macranthoidin B and dipsacoside B. [Result] The optimal HP-20 purification conditions inducted: a concentration of sample liquid at 4.8 mg/mi, a sample volume of 2 BV, an adsorption flow rate at 1.5 BV/h, an ethanol concentration for desorption at 60%, a desorption volume of 3 BV, and a desorption flow rate at 1.5 BV/h. Total saponins were then separated by an HP-SS macroporous resin column which was eluted sequentially by water, 20% ethanol, 30% ethariol, 40% ethanol and 50% ethanol. Two purified compounds were obtained in fractions eluted by 40% ethanol and 50% ethanol, respectively. The two compounds were identified as macranthoidin B and dipsacoside B by 13C and 1H nuclear magnetic resonance spectroscopy. [Conclusion] The combination of HP-20 and HP-SS macroporous resin could efficiently separate macranthoidin B and dipsacoside B from Flos Lonicerae.
- Publication
Agricultural Science & Technology, 2016, Vol 17, Issue 4, p765
- ISSN
1009-4229
- Publication type
Academic Journal