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- Title
PLANT GENETIC RESOURCES ASSAY FOR ABIOTIC STRESS-TOLERANT TRAITS USING TISSUE CULTURE TECHNIQUES: A REVIEW.
- Authors
JATHUNARACHCHI, A. S.; PERERA, P. I. P.; SALGADOE, A. S. A.
- Abstract
Considering that identifying the right progenitors among available genetic resources is one of the key factors that determine the efficiency of crop improvement programs, screening germplasm is important. A broad range of different methodological approaches for selection is available. Tissue culture-based in vitro selection is one of the most high-throughput, efficient, and cost-effective tools for screening stress-tolerant plants. In the absence of external environmental threats, the testing factor is altered, whereas all other conditions are kept similar. The reduced space requirement for screening a large number of germplasm is also advantageous. This technique has been efficiently used to screen genetic resources in different aspects and for tolerance to abiotic factors, such as drought, temperature, and salt stresses and low nutrient levels. This review summarizes the information on the tested stresses and their levels under in vitro conditions, the threshold levels for screening, the types of explants, the compositions of the media, the parameters recorded, the performances of different crops under the stressed conditions, and the selected genotypes with resistant/tolerant traits.
- Publication
SABRAO Journal of Breeding & Genetics, 2021, Vol 53, Issue 3, p334
- ISSN
1029-7073
- Publication type
Academic Journal