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- Title
Spinal Manipulative Therapy Depresses Hyperalgesia and Abnormal Expression of c-Fos and PKCγ in the Dorsal Horn of the Spinal Cord in Rats With Lumbar Intervertebral Foramen Inflammation.
- Authors
Xue-Jun Song; Xue-Song Song; Rupert, Ronald L.
- Abstract
Introduction: The authors have recently shown that Activator-assisted spinal manipulative therapy (SMT) significantly reduces pain and hyperalgesia following intervertebral foramen (IVF) inflammation in rats. This study further examined effects of SMT on IVF inflammation-induced expression of c-Fos and protein kinase C gamma (PKCgamma) representing neural hyperactivity and central sensitization in the spinal dorsal horn (DH). Methods: Experiments were performed on 72 adult, male Sprague-Dawley rats. IVF inflammation was produced by injecting inflammatory soup (IS, 10 µL) into IVF at L5. SMT was applied to the spinous process of L5 and L6. Thermal hyperalgesia was evaluated by measuring changes in latency of hindpaw foot withdrawal to radiant heat stimulation. DAB immunostaining for c-Foe and fluorescence immunohistochemistry for PKCgamma were used to stain c-Fos and PKCgamma expression. Results: The results showed that IVF inflammation produced significant hyperalgesia and increased expression of early gene c-Fos and PKCgamma. Fos protein and PKCgamma were rapidly increased in DH in response to IVF inflammation. Increase in expression of c-Foe and PKCgamma began on day 1 to day 21, the last examined point, and peaked at 5 and 7 days after IVF inflammation. SMT significantly reduced hyperalgesia and the increased expression of c-Fos from day 5 to day 21, and PKCgamma at day 5 and 7. The present studies confirm the authors' previous finding that SMT significantly reduces severity and shortens duration of pain and hyperalgesia caused by lumbar IVF inflammation, and further demonstrate that SMT can reduce the neural hyperactivity and central sensitization exhibited by c-Fos and PKCgamma in DH. Conclusion: It was concluded that SMT can effectively reduce IVF inflammation-lnduced pain and hyperalgesia via inhibition of central sensitization in addition to faster elimination of the dorsal root ganglion inflammation.
- Publication
Journal of Chiropractic Education (Association of Chiropractic Colleges), 2008, Vol 22, Issue 1, p77
- ISSN
1042-5055
- Publication type
Academic Journal