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- Title
Association of Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) Gene Expression and Caspase Activity in Normal Nasopharyngeal Cell.
- Authors
Mustapha, Mohd Aminudin; Sai-Peng Sim
- Abstract
Objective: To assess LMP1 gene expression-induced apoptosis in normal nasopharyngeal cells by measuring caspase activity. Material and Methods: LMP1 gene was subcloned into pTracer and pcDNA vector, producing pTracer-LMP1 and pcDNA- LMP1 expression plasmids. The plasmids were then transfected into normal nasopharyngeal cells. LMP1 gene expression-induced apoptosis was accessed by measuring Caspase activities using Caspase-Glo ® 3/7 Assay kit following the manufacturer's protocol. The luminescence intensity was measured by microplate reader. Association of LMP1 gene expression with caspase activation was analysed by independent Sample T test. Results: LMP1 gene expression in normal nasopharyngeal cells is significantly associated with higher caspase activity of apoptosis compare to the vector control with t(-2.142), p value of 0.03. Conclusion: Our results show that, there is association of LMP1 gene expression and caspase activity level in normal nasopharyngeal cell thus support that LMP1 gene expression is involved in apoptosis induction. This in turn suggests that the apoptosis process is potentially involved in the carcinogenesis of nasopharyngeal carcinoma.
- Publication
International Medical Journal, 2021, Vol 28, Issue 5, p524
- ISSN
1341-2051
- Publication type
Academic Journal