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- Title
Anti-Inflammatory Activities of Digested Green Curry Paste in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients.
- Authors
Sirikanokvilai, P.; Kriengsinyos, W.; Nantiruj, K.; Muangnoi, C.; Chingsuwanroj, P.; Praengam, K.; Tuntipopipat, S.
- Abstract
Introduction: Several individual spices have been shown to have anti-inflammatory activity. However, the anti-inflammatory activities of mixed spices consumed as components of habitual diets are limited. We aimed to assess the anti-inflammatory activities of ‘Green curry paste ‘(GCP) consisting of nine different spices in cell culture models. Methods: Non-toxic diluted bioaccessible fraction generated by simulated digestion of GCP was added to the apical compartment of Caco-2 cells grown on bicameral inserts for 4 h. Basolateral media (bioavailable fraction) was added to cultures of peripheral blood mononuclear cells (PBMC) from 12 patients with rheumatoid arthritis in the absence or presence of lipopolysaccharide (LPS). Cytokines in culture media were quantified by ELISA. Caco-2 cells also were activated with IL-1βfollowing exposure to the bioaccessible fraction of GCP to determine its effect on chemokine expression. Results: Spontaneous and LPS-induced secretions of IL-6 were significantly decreased when PBMC were exposed to the basolateral medium from Caco-2 cell cultures incubated with the bioaccessible fraction of GCP compared to control cultures. In a similar procedure, secretions of TNF-and IL-8 were also decreased, but the changes were not significantly different from the controls. Pre-treatment of Caco-2 cells with the bioaccessible fraction of GCP also significantly suppressed IL-1β-induced expression of IL-8 (62% protein and 63% mRNA) and MCP-1 (47% protein and 51% mRNA) (p<0.05). Conclusion: These results suggest the use of GCP as a food component towards attenuating the severity of inflammatory conditions.
- Publication
Malaysian Journal of Nutrition, 2014, Vol 20, Issue 2, p271
- ISSN
1394-035X
- Publication type
Academic Journal