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- Title
IN VITRO CHARACTERIZATION AND SAFETY OF THE PROBIOTIC STRAIN LACTOBACILLUS REUTERI CARDIOVIVA NCIMB 30242.
- Authors
Branton, W. B.; Jones, M. L.; Tomaro-Duchesneau, C.; Martoni, C. J.; Prakash, S.
- Abstract
In-vitro characterization and safety evaluations were performed for Lactobacillus reuteri cardioviva NCIMB 30242 using molecular and metabolic analysis techniques to investigate the safety of the strain for human consumption. First, the identity of the strain was confirmed using a combination of metabolic profiling and 16s rDNA sequencing techniques. The genome of the strain was sequenced by a shotgun sequencing strategy and annotated using RAST software. Results show that the strain does not have antimicrobial resistance genes or other virulence factors uncommon to other probiotic Lactobacilli or associated with mobile genetic elements. This genetic analysis was further confirmed by phenotypic testing. The minimum inhibitory concentration (MIC) of a panel of antibiotics showed that this strain is susceptible to the 8 antibiotics tested. The ability of the strain to produce potentially harmful by-products and antimicrobial compounds was also tested. Results show that the strain does not produce biogenic amines above the detection limits for plate screening and HPLC based methods. In addition, the strain was shown to produce lactic acid and hydrogen peroxide and did not show bacteriocin activity or reuterin production. These results confirm that Lactobacillus reuteri cardioviva NCIMB 30242 is a safe probiotic strain; however, in vivo validation is required.
- Publication
International Journal of Probiotics & Prebiotics, 2011, Vol 6, Issue 1, p1
- ISSN
1555-1431
- Publication type
Academic Journal