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- Title
Effect of Ulinastatin on liver regeneration and energy metabolism after major hepatectomy combined with ischemia reperfusion injury.
- Authors
TAN Jing; ZHU Yu-lin; ZHOU Rong-sheng; ZHANG Xiao-qi; ZHAO Ge; LIU Qi-ning
- Abstract
Objective To investigate the effect of ulinastatin (UTI) pretreatment on liver regeneration and energy metabolism after 70% hepatectomy combined wíth ischemia reperfuison injury in rats. Methods Totally 120 healthy clean grade male Sprague-Dawley rats weighting 230~280 g were selected and aligned into three groups randomly: imple partial hepatectomy (PH) group, partial hepatectomy combined with ischemia reperfuison (PHIR) group and ulinastatin (UTI) group, with forty rats in each. Rats in each group had the left and middle liver lobes resected, but the remnant lobes (right and caudate lobes) were treated differently as follows: the blood flow to the reserved lobes was not occluded in PH group, but occluded for 30 minutes in PHIR group, and then reperfused. UTI group had the same liver resection and blood flow occlussion as PHIR group except for administration of ulinastatin 50 000 U/kg by i.v. injection via caudal vein. Inferior vena cava blood and the reserved liver tissue were collected to measure serum alanine transaminase (ALT) and aspartate aminotransferase (AST), apoptois index (AI), regenerated liver weight, PCNA staining as well as ATP, ADP and AMP contents. Results Serum ALT and AST activites and AI level were lower, and the regenerated liver weight and poitive PCNA staining as well as ATP content and energy charge were significantly higher inUTI group than in PHIR group (P<0.05). Conclusion ulinastatin can promote liver regeneration after major hepatectomy combined with ischemia reperfuison injury, which is possibly related to maintaining energy metabolism.
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2014, Vol 35, Issue 1, p89
- ISSN
1671-8259
- Publication type
Academic Journal
- DOI
10.7652/jdyxb201401020