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- Title
Effect of Mono-(2-ethylhexyl) Phthalate on Steroidogenesis in Human Granulosa Cells.
- Authors
CAI Xiao-hui; SHI Cheng; SHEN Huan
- Abstract
Objective: To investigate the toxic effect of mono-(2-ethylhexyl) phthalate (MEHP) on steroidogenesis in human granulosa cells(GCs). Methods: Follicular fluid were collected from six patients undergoing in vitro fertilization. GCs were extracted and cultured in vitro for 72 hours, and then treated with MEHP (25, 300, 500 µmol/L). After treatment for 48 hours, the expression levels of CYP11A1, PPAR- γ, CYP19A1 and 3β-HSD mRNAs were determined by real-time PCR. Results: The expression of CYP19A1 mRNA in GCs was significantly down-regulated by MEHP treatment of 300 µmol/L and 500 µmol/L (P<0.05), and the expression of CYP11A1 was significantly down-regulated by MEHP treatment of 500 µmol/L(P<0.05). Also, the expression of 3β-HSD mRNA was decreased by 500 µmol/L MEHP treatment (P<0.05), while PPAR-γ expression was increased by 300 µmol/L and 500 µmol/L MEHP treatment (P<0.05). Conclusions' MEHP at higher doses affects steroidogenesis in GCs by down-regulating the expressions of steroid biosynthesis enzymes CYP11A1, CYP11A1 and 3β-HSD, and by up-regulating the expression of PPAR-γ.
- Publication
Journal of International Reproductive Health/Family Planning, 2015, Vol 34, Issue 3, p189
- ISSN
1674-1889
- Publication type
Academic Journal