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- Title
Anticancer Potential of Angucyclone Polyketides from Streptomyces carlesensis Strain DSD011 against Human Lung, Colorectal, Breast, and Ovarian Cancer Cell Lines.
- Authors
Bayog, Leah Katrine; Octaviano, May; Amago, Diana; Aguadera, Angelica Marie; Saludes, Jonel; Dalisay, Doralyn
- Abstract
Introduction: Cancer morbidity and mortality have been increasing at an alarming rate, urging drug discovery programs to discover potential anticancer compounds from natural products. The Philippine marine sediments harbors bacteria that produce metabolites with potent bioactivities, especially those that belong to the genus Streptomyces. This study aimed to determine the antiproliferative activity of Streptomyces carlesensis DSD011T isolated from the marine sediments of Islas de Gigantes, Iloilo, Philippines against four cancer cell lines. Method: Streptomyces carlesensis DSD011T extract was purified by HPLC to yield semi-pure and pure compounds, which were characterized by spectroscopic analysis (HRMS and NMR). These compounds were tested using MTT assay against cells of human colorectal cancer (HCT-116), human ovarian cancer (A2780), human breast cancer (MCF-7), and human lung cancer (A549) at final concentrations of 100 and 10 µg/mL. The positive controls used were 5-fluorouracil, cisplatin, tamoxifen, and doxorubicin hydrochloride for HCT-116, A2780, MCF-7, and A549, respectively. Results: Results showed that several fractions demonstrated cancer cell growth inhibitory activity. Specifically, fraction DSD011G-6I2H41 exerted the most notable antiproliferative activity at both testing concentrations. At 100 µg/mL, the fraction showed 85%, 92%, 83%, and 78% growth inhibition against HCT-116, A2780, MCF-7, and A549, respectively. Conversely, the fraction demonstrated 84%, 97%, 92%, and 69% growth inhibitory activity at 10 µg/mL against HCT-116, A2780, MCF-7, and A549, respectively. The MS and NMR analysis indicate that these fractions contain angucycline polyketide compounds, known to have anticancer activities. Additionally, unpaired t-test with Welch correction showed that there is no significant difference (p=0.05) between the bioactivities exhibited by fraction DSD011G6I2H41 at concentrations 100 and 10 µg/mL, indicating that the tested fraction has high antiproliferative activity even at a low testing concentration. Conclusion: Streptomyces carlesensis DSD011T isolated from the marine sediments of Islas de Gigantes is a promising candidate for anticancer drug discovery.
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p19
- ISSN
1675-8544
- Publication type
Academic Journal