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- Title
Biological activities, total phenolic compound and TLC fingerprint of Eclipta prostrata (L.) L., Sphagneticola calendulacea (L.) Pruski and Sphagneticola trilobata (L.) Pruski.
- Authors
Sangkaram, Nisarat; Sangthog, Bunleo; Rattarm, Ruchilak
- Abstract
Eclipta prostrata (L.) L., Sphagneticola calendulacea (L.) Pruski and S. trilobata (L.) Pruski are the member of Asteraceae family. Similar characteristics may be misleading and misused. This study aimed to compare biological activities of ethanolic and water extracts of these plants. Antibacterial activity was assessed using broth dilution method for MIC and MBC determination, anti-inflammatory activity using lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells, antioxidant activity using DPPH radical scavenging assay, total phenolic contents using Folin-Ciocalteu reagent and chemical constituents were investigated by TLC their fingerprint. All the tested extracts exhibited low antibacterial effects against S. aureus, P. aeruginosa and MRSA. The ethanolic extracts showed significantly stronger inhibition on nitric oxide production than did water extracts and the standard anti-inflammatory medicine, diclofenac. Among them, S. trilobata had greatest inhibitory activity with IC50 33.43±4.76 µg/ml. The ethanolic extracts of S. calendulacea showed the highest antioxidant activity and phenolic content with IC50 of 43.48±1.00 µg/ml and 55.46±7.18 mg GAE/g crude extract, respectively. TLC fingerprint of the ethanolic extracts of these three plants were identified. In conclusion, W. chinensis, S. calendulacea and S. trilobata are different as assessed by both their biological activities and their chemical identification by TLC.
- Publication
Journal of Science & Technology MSU, 2021, Vol 40, Issue 4, p341
- ISSN
1686-9664
- Publication type
Academic Journal