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- Title
Cytotoxic Effects and Osteogenic Activity of Calcium Sulfate with and without Recombinant Human Bone Morphogenetic Protein 2 and Nano-Hydroxyapatite Adjacent to MG-63 Cell Line.
- Authors
Ghorbanzadeh1,2, Abdollah; Aminsobhani, Mohsen; Khoshzaban, Ahad; Abbaszadeh, Armin; Bolhari, Behnam; Ghorbanzadeh, Atiyeh; Shamshiri, Ahmad Reza
- Abstract
Objectives: The aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhBMP2) and ano-hydroxyapatite (n- HA) adjacent to MG-63 cell line. Materials and Methods: To assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay was used. Alkaline phosphatase (ALP) activity and osteogenic activity were evaluated using Alizarin red and the von Kossa staining and analyzed by one-way ANOVA followed by Tukey's post hoc test. Results: The n-HA/CS mixture significantly promoted cell growth in comparison to pure calcium sulfate (CS). Moreover, addition of rhBMP2 to CS (P=0.02) and also mixing CS with n-HA led to further increase in extracellular calcium production and ALP activity (P=0.03). Conclusion: This in vitro study indicates that a scaffold material in combination with an osteoinductive material is effective for bone matrix formation.
- Publication
Journal of Dentistry of Tehran University of Medical Sciences, 2015, Vol 12, Issue 5, p352
- ISSN
1735-2150
- Publication type
Academic Journal