We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
شيوع مقاومت آنتي بيوتيكي استافيلوكوك اورئوس مقاوم به متي سيلين و تعيين ژن مقاومت به آمينوگليكوزيد به روش PCR در بيمارستان هاي ساري و تهران.
- Authors
مهديون, سيد محسن; آهنجان, محمد; گودرزي, مهدي; رضايي, راضيه
- Abstract
Background and purpose: Staphylococcus aureus is one of the major causes of community and hospital-acquired infections. Aminoglycosides are potent bactericidal agents that are often used in combination with beta-lactam or glycopeptide in treatment of staphylococcal infections. The aim of this study was to investigate the prevalence of the aminoglycoside modifying enzyme aac(6')-Ie/aph(2") genes and mecA. Materials and methods: In current study, 174 clinical isolates of MRSA from different clinical specimens were tested for antibiotic susceptibility using the Kirby Bauer method. MRSA isolates were detected by disc diffusion method using 1 ug oxacillin and 30 ug cefoxitin discs. Then, MRSA strains were further analyzed for detection of mecA and aminoglycoside resistant aac (6')-Ie/aph (2") genes by PCR. Results: All strains were resistant to oxacillin and cefoxitin. The highest and lowest resistances were found against erythromycin (85.1%) and trimethoprim/sulfamethoxazole (24.7%), respectively. In PCR, mecA gene was detected in 100% of the strains and 77% of the strains were found to harbor aac(6')/aph(2")-Ia gene. Conclusion: According to this study, the prevalence of aminoglycosides resistant genes is increasing in MRSA isolates.
- Publication
Journal of Mazandaran University of Medical Sciences (JMUMS), 2015, Vol 25, Issue 128, p97
- ISSN
1735-9260
- Publication type
Academic Journal