We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Roasted Coffee Extract Inhibited Cholesterol Absorption by Interfering with Lipid Digestion Enzyme and Micellar Cholesterol Properties.
- Authors
Atcharaporn Ontawong; Acharaporn Duangjai
- Abstract
Background/Aims Coffea arabica L. (Rubiaceae) infusion (common plant name "coffee") is one of the most widely consumed beverages. Roasting coffee transforms the chemical and physical properties of green coffee beans into roasted coffee products. Among its major constituents including caffeine and chlorogenic acid, caffeine has been shown to decrease lipogenesis genes expression resulted in the reduction of hepatic triglyceride and cholesterol accumulation. Thus, roasted coffee could also have a potential for lipid lowering effects. However, it remains uncleared whether roasted coffee extract (RCE) has anti- hyperlipidemic effects. Therefore, the aim of this study was to investigate the effect of RCE on lipid digestion enzyme and micellar cholesterol formation. Methods Roasted coffee was extract by ultrasound-assisted extraction method with various time (5, 10 and 20 minutes). The inhibitory property of RCE on pancreatic lipase was determined using fluorometric method. The percentage of lipase enzyme inhibition was calculated as compared to the initial activity measured in the absence of pancreatic lipase enzyme inhibitor. In addition, cholesterol micelles particle size and solubility were quantified using particle size analyzer and colorimetric assay, respectively. Results RCE in all extraction time at 100 mg/mL increased mixed micelle particle sizes. Interestingly, ultrasound-assisted extraction of roast coffee for 5 minutes highly elevated cholesterol micelle size. Furthermore, RCE significantly reduced cholesterol solubility and also showed strong inhibitory effects against pancreatic lipase enzyme. Conclusions RCE has a lipid-lowering effect in in vitro by interfering with pancreatic lipase enzyme activity and cholesterol micellar complex formation. Accordingly, RCE could be developed as nutraceutical product to prevent dyslipidemia-induced obesity and insulin resistance.
- Publication
Gut & Liver, 2019, Vol 13, Issue 6(suppl. 1), p182
- ISSN
1976-2283
- Publication type
Academic Journal