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- Title
Cellulolytic activity of tropical marine rotifer (Brachionus rotundiformis).
- Authors
Paulines, Mellie June S.; Pahila, Ida G.; Nillos, Mae Grace G.; Primavera, Karlo H.
- Abstract
Cellulose degrading enzymes are important in agriculture, aquaculture, feeds and food, drugs and biotechnology. Furthermore, the high cost of these enzymes and large market potential especially for bioethanol production provides a motivation to search for new enzyme source. This study is the first to characterize the cellulose degrading enzyme in the marine rotifer, Brachionus rotundiformis. The influence of pH, temperature and substrate concentration on cellulolytic activity was assessed using a water soluble substrate carboxymethyl cellulose (CMC), to determine the conditions for maximum enzyme activity. Maximum catalytic activity was at pH 5.0. The hydrolysis is effective in acid digestion and is in the range of intestinal pH of the rotifer. The observed optimum temperature for substrate hydrolysis (30.0°C) is in the temperature range (28.0-35.0 °C) of the rearing medium for the organism. Substrate concentration - activity plot revealed of Km of 2.5 mg CMC/mL and a Vmax of 178.6 µg glucose mg protein-1 hr-1 . In vitro hydrolysis of pure cellulose (filter paper) established that the crude enzyme has cellulolytic activity. It is highly recommended to pursue enzyme purification and further characterization using the protocol established in this study and be tested for the hydrolysis of lignocellulose plant material for fermentable sugar production.
- Publication
Extreme Life, Biospeology & Astrobiology, 2013, Vol 5, Issue 1, p21
- ISSN
2066-7671
- Publication type
Academic Journal