We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
同位素内标-超高效液相色谱-高分辨质谱法 测定酒醅中氨基甲酸乙酯.
- Authors
尹艳艳; 黎露露; 杨军林; 冯小兵; 蒋力力; 田栋伟; 尤小龙; 程平言
- Abstract
Objective To establish an analytical method for ethyl carbamate (EC) in fermented grains by isotope internal standard method combined with ultra performance liquid chromatography-high resolution mass spectrometry. Methods Fermented grains were extracted by ultrapure water, filtered after centrifugation. The 0.10% formic acid aqueous solution and methanol solution were used as mobile phase for gradient elution. The samples were separated by Hypersil GOLD Amino chromatographic column; the internal standard method with full mass scan mode was used for quantitative analysis in electrospray positive ion mode. Results The matrix interference was effectively compensated by internal standard quantitative method. The calibration curve of EC showed good linearity in the range of 4.00‒300.00 μg/L (r2 =0.9993), the limit of quantification was 6.25 μg/kg, and the limit of detection was 3.75 μg/kg. The recoveries of the actual samples ranged from 85.17%‒97.40%, and range of relative standard deviations were 1.12%‒4.11%. Conclusion The linearity, accuracy and precision of this method meet the requirements of GB/T 27417—2017 Guidelines for the confirmation and verification of chemical analysis methods, and it is suitable for the detection of EC in fermented grains.
- Publication
Journal of Food Safety & Quality, 2022, Vol 13, Issue 23, p7807
- ISSN
2095-0381
- Publication type
Academic Journal