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- Title
Protective role of meteoroid-like peptide and exercise in cardiac ischemia reperfusion injury.
- Authors
YAKINBAŞ, Tuğçe; ÖNALAN, Ebru; TEKTEMUR, Ahmet; ONAT, Elif; KAÇAR, Emine; KULOĞLU, Tuncay; ŞAHNA, Engin; ÖZERCAN, İbrahim
- Abstract
Myocardial infarction (MI) is a heart disease with a high mortality and morbidity rate. Disturbances in mitochondrial function in heart diseases lead to an increase in ROS levels (Chistiakov et al., 2017). Exercise reduces cardiac ROS production in the mitochondria of the heart (Starnes 2007). Meteorin-like peptide (Metrnl) synthesized from muscle tissue is known to be associated with exercise. The aim of our study was to investigate the relationships between Metrnl, exercise and mitochondrial biogenesis (MB) in ischemia-reperfusion (I\R) injury. Metrnl levels in human control (n=200) and MI serum samples (n = 200) were assessed using ELISA assay. Wistar albino rats were divided into six groups as control, I\R, exercise (E), I\R + E, I\R + Metrn1 and I\R+E + Metrn1 as six animals in each group. A 6-week treadmill running exercise was performed for the exercise groups. Expression levels of Metrn1 and MB related genes in heart and muscle tissues were evaluated by qRT-PCR. The effect of Metrnl on vascular contractions was determined by an isolated organ bath experiment. Current study was determined that human serum Metrn1 levels did not change in MI patients compared to the control. According to the control group in rats, heart Metrnl expression decreased significantly in the IR group. Metrn1 levels were normalized the decrease in all exercise groups by the exercise. Metrn1 peptide levels in muscle tissue were significantly increased in the exercise compared to control, but there was no significant change in IR groups. It was determined that MB-related gene expressions were significantly altered in heart and muscle in all groups than control. Metrnl was significantly increased the vascular contractions in an isolated organ bath experiment. In conclusion, present study was observed that the protective feature of the exercise in I/R was related to MB. It was found that Metrn1 decreased in IR damage and that the exercise normalized this decrease. In particular, Metrnl was found to have protective properties in IR by increasing Tfam levels in muscle and heart tissues.
- Publication
Journal of Cellular Neuroscience & Oxidative Stress, 2018, Vol 10, Issue 2, p756
- ISSN
2149-7222
- Publication type
Academic Journal