We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Simultaneous Determination of Six Constituents in Cangbaiqutong Capsule by HPLC Wavelength Switching Technique.
- Authors
Jing WU; Hongyan LIU; Bo YU; Zhong JIA
- Abstract
[Objectives] To establish a high performance liquid chromatography (HPLC) wavelength switching method for the simultaneous determination of content of six constituents (phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and pae-onol) in Cangbaiqutong capsules, and provide a scientific basis for the comprehensive evaluation of the quality of Cangbaiqutong capsule. [Methods] The chromatographic column of Waters XSELECT CSH-C18 (4.6 mm x 150 mm, 5 µm), the mobile phase of acetonitrile-0.1% phosphate solution, gradient elution (0-15 min, 10% - 18% A; 15 - 30 min, 18% - 50% A; 30 - 35 min, 50% - 10% A); the flow rate of 1.0 mL/min, wavelength switching of 284 (0 - 7 min, phellodendrine), 274 (7 - 10 min, gentiopicrin), 230 (10 - 14 min, paeoniflorin) and 274 nm (14 - 35 min, tetrandrine, berberine hydrochloride, paeonol), the injection volume of 10 µL. [ Results] There was a good linear relationship between the area of chromatographic peak and the injection volume of phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol in the range of 0.150 - 1.504, 0.768 - 7.680, 1.096 - 10.960, 0.220 - 2.200, 0.296 - 2.956, 0.0345 - 0.345 µg, respectively; the average recovery rates (n = 6) were 98.3%, 99.2%, 98.8%, 98.8%, 99.1% and 98.2%, respectively; the RSD value was 1.32%, 1.46%, 1.08%, 1.31%, 1.26% and 1.21%, respectively. [Conclusions] The method can be used to determine many kinds of constituents at the same time, and the operation is simple, accurate and reproducible, and can be used for the quality control of compound Cangbaiqutong capsules.
- Publication
Medicinal Plant, 2019, Vol 10, Issue 4, p20
- ISSN
2152-3924
- Publication type
Academic Journal
- DOI
10.19600/j.cnki.issn2152-3924.2019.04.005