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- Title
تولید و بهینهسازی نانوسامانههای کیتوزان برای حمل فراکسیون پپتیدی حاصل از (Litopenaeus vannamei) هیدرولیز آنزیمی ضایعات سر میگوی وانامی.
- Authors
قاسم رشیدیان; عبدالمحمد عابدی&; مریم نیکخواه
- Abstract
The aim of this study was to investigate optimization of encapsulation of peptide fraction smaller than 10 kDa obtained from enzymatic hydrolysis of shrimp head wastes (Litopenaeus vannamei) by chitosan. Based on ion gelation method (chitosan-tripolyphosphate) encapsulated was optimized based on different ratios of chitosan: triphosphate and different concentrations of peptide fractions including 1, 5 and 10 mg/ml. Based on the results, alkalase enzyme hydrolyzed shrimp head wastes with 57±2.37 degree of hydrolysis and according to the conditions of this experiment, the peptide chain length was 1.175 and molecular weight was close to 10 kDa. The particle size varied in different concentrations and treatments, and ranged from 30 to 150 nm, with the best results from encapsulation optimization in a 2: 1 ratio of chitosan to TPP and Concentration of 10 mg/ml. The PDI, zeta potential, and size of nanocapsules in the optimal conditions were 0.375, 2020 and 30.13 nm, respectively, and storage conditions at -20 °C had limited effect on the quality of nanocapsules. Our findings suggest that chitosan can be well used for nanocapsulation of peptide fractions smaller than ten kDa with an efficiency of 91.04±0.18%.
- Publication
Journal of Fisheries Science & Technology, 2021, Vol 10, Issue 2, p189
- ISSN
2322-5513
- Publication type
Academic Journal