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- Title
Effect of immobilization on the enantioselective esterification of ibuprofen using lipase from Candida cylinderacea.
- Authors
Krishnan, Sankarasubramanian; Madhumitha, Srinivas Raj; Bharathi, Periyasamy; Ravi, Thengungal Kochupapy
- Abstract
The S-enantiomer of ibuprofen [2-(4-isobutylphenyl) propionic acid] contributes to most of the racemate's anti-inflammatory activity. Reports suggest that, in the case of in vitro synthesis of prostaglandins, the S-(+) enantiomer is almost 160 times more active than its antipode. In order to circumvent the undesired effects of the R-enantiomer of the profens, it is ideal to use the pure S-enantiomer. The ester of ibuprofen has also been reported to be used as a pro-drug. In this context the stereoselective esterification of ibuprofen was carried out using immobilized lipase from Candida cylinderacea using chitosan-xanthan and calcium alginate respectively. The effect of the immobilized lipase and free lipase on enantioselective esterification of ibuprofen using amyl alcohol as primary alcohol and isooctane as a solvent was also studied. The conversion of ibuprofen to its ester was monitored by thin layer chromatography (TLC) and high performance TLC (HPTLC) methods. Our result showed that, free lipase exhibited higher esterification than immobilized lipase. Free lipase from Candida cylindracea showed 24% more activity than lipase immobilized in chitosan-xanthan and 36% more activity than lipase immobilized in calcium alginate.
- Publication
Manipal Journal of Pharmaceutical Sciences, 2017, Vol 3, Issue 1, p15
- ISSN
2455-3735
- Publication type
Academic Journal