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- Title
EP389 THE ANTI-BIOFILM ACTIVITY OF ANTIMICROBIAL DRESSINGS USING INCREASINGLY STRINGENT IN VITRO MODELS.
- Authors
Metcalf, Dan; Meredith, Kate
- Abstract
Aim: To evaluate the anti-biofilm activity of 4 antimicrobial dressings using a series of increasingly relevant and complex in vitro biofilm tests. Method: The Centers for Disease Control & Prevention (CDC) biofilm reactor model with single-species standard strains, antibiotic-resistant strains and polymicrobial biofilm (Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) was used to assess anti-biofilm activity of test dressings. Test dressings included: an anti-biofilm ionic silver carboxymethylcellulose (CMC) dressing (ABS-CMC); a calcium alginate/CMC dressing containing ionic silver (CA-Ag); a polyurethane foam dressing with proprietary ionic silver complex (Ag-Foam); a hydrated biocellulose dressing containing polyhexamethylene biguanide (BC-PHMB). Test dressings were applied for 24 hours and surviving biofilm cells were enumerated. Results: Single-species biofilm testing showed that only ABS-CMC reduced all 3 single-species biofilms (S. aureus, P. aeruginosa and C. albicans) by at least 4 log10. For resistant strains, ABS-CMC and Ag-Foam reduced counts for both MRSA and resistant P. aeruginosa single-species biofilm by >3 log10 and BC-PHMB reduced counts of MRSA by 3 log10. Against polymicrobial biofilm, ABS-CMC completely eradicated all microorganisms (6.5 log10 reduction). In contrast, CA-Ag, Ag-Foam and BC-PHMB reduced total microorganisms by 3.0 log10, 4.3 log10 and 3.9 log10 respectively. Conclusion: ABS-CMC dressing resulted in enhanced activity against single-species, antibiotic-resistant and polymicrobial biofilm cultured in the CDC biofilm reactor compared with the other 3 dressings tested. To be effective against polymicrobial biofilm, which is observed clincially and a known barrier to wound healing, it is important that wound dressings contain additional antibiofilm properties as well as antiseptic agents.
- Publication
Journal of Wound Management, 2023, Vol 24, Issue 2, p248
- ISSN
2788-5771
- Publication type
Academic Journal